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11,12-leukotriene A4: synthesis and metabolism
I Miki1, S Kitamura, T Shimizu
1Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
Advances in Prostaglandin, Thromboxane, and Leukotriene Research
|January 1, 1989
Abstract:
11(S),12(S)-oxido-5Z,7E,9E,14Z-eicosatetraenoic acid (11,12-LTA4) was chemically synthesized from 12-hydroperoxy-eicosatetraenoic acid (12-HPETE). 11,12-LTA4 methyl ester was nonenzymically hydrolyzed to at least four products. These products were identified to be epimers of 5,12(S)-dihydroxy-eicosatetraenoic acid methyl ester (5,12(S)-diHETE-Me) and epimers of 11,12-diHETE-Me. In addition to the above nonenzymic products, 11,12-LTA4 was converted to 11,12-LTC4 by the rat liver glutathione S-transferase, and to an isomer of 11,12-diHETE by homogenates of the guinea pig adrenal gland and liver.