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A novel controlled-release system for antibacterial enzyme lysostaphin delivery using hydroxyapatite/chitosan
Bai Xue1, Cheng Zhang2, Yihan Wang3
1State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, 200433, PR China.
Plos One
|December 3, 2014
Summary
This study developed a novel injectable bone cement for controlled release of lysostaphin (LSN), an antibacterial protein. The developed bone cement demonstrated effective protein delivery and retained antibacterial activity against MRSA.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Orthopedic Surgery
Background:
- Bone defects and infections pose significant clinical challenges.
- Effective delivery of antibacterial agents is crucial for treating bone infections.
- Current treatments often struggle with controlled drug release and localized efficacy.
Purpose of the Study:
- To develop a self-setting, injectable, porous bone cement for controlled release of lysostaphin (LSN).
- To evaluate the physicochemical properties, protein release kinetics, biocompatibility, and antibacterial efficacy of the developed bone cement.
Main Methods:
- Preparation of hydroxyapatite/chitosan (HA/CS) composite scaffold-based bone cement.
- Characterization of setting parameters, microstructure, and composition (SEM, XRD, FTIR).
- In vitro and in vivo assessment of LSN release, biocompatibility (MTT assay, H&E staining), and antibacterial activity against MRSA.
Main Results:
- The bone cement exhibited a final setting time of ~97 min, ~34% porosity, and good biocompatibility.
- In vitro release showed ~94% LSN release by day 8; in vivo tracking indicated slower release than free LSN.
- The LSN-loaded cement demonstrated potent antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA).
Conclusions:
- The developed LSN-loaded bone cement provides controlled and effective protein delivery, retaining protein activity.
- This injectable, porous bone cement holds potential as a bone substitute and drug delivery system for treating bone defects and infections.
