Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A model for hydropathy-based peptide interactions.

G Markus1, G L Tritsch, R Parthasarathy

  • 1Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.

Archives of Biochemistry and Biophysics
|August 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comment on "Does pelvic fixation impact reoperation outcomes for neuromuscular scoliosis surgery? A 10-year matched cohort analysis".

Spine deformity·2026
Same author

Vestibular schwannoma unveiled by pregnancy: A case report and literature review.

European journal of obstetrics, gynecology, and reproductive biology·2024
Same author

Postoperative respiratory depression caused by iatrogenic hypermagnesaemia.

Anaesthesia reports·2022
Same author

Design and development of heater control circuit without temperature sensor for monitoring hydrogen in argon.

The Review of scientific instruments·2022
Same author

Bio inspired growth factor loaded self assembling peptide nano hydrogel for chronic wound healing.

International journal of biological macromolecules·2021
Same author

An Unusual Association of Renal Cell Carcinoma and Renal Malakoplakia with Focal Segmental Glomerulosclerosis in an Elderly Patient.

Indian journal of nephrology·2019

Two peptides derived from DNA reading frames form strong complexes. Optimal binding occurs when peptides align parallel or antiparallel, driven by hydropathic complementarity between amino acids.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Two peptides can be generated from a single noncoding DNA strand by reading in either the 5' to 3' or 3' to 5' direction.
  • Previous research indicated that these peptides form strong complexes with natural peptides, such as ACTH, with hydropathic complementarity proposed as the interaction basis.

Purpose of the Study:

  • To investigate the specific alignment requirements for strong hydropathic complementarity between peptides derived from opposite DNA reading frames.
  • To elucidate the structural model explaining how hydropathic complementarity drives peptide-peptide interactions.

Main Methods:

  • Analysis of peptide alignments based on DNA codon complementarity and amino acid hydropathy.
  • Development of a molecular model to describe the interaction interface between complementary peptides.

Related Experiment Videos

Main Results:

  • Strong hydropathic complementarity is achieved only when the 3'-to-5' derived peptide is aligned parallel to the natural peptide, or the opposite-reading peptide is antiparallel.
  • Other alignments, including staggered ones, abolish complementarity.
  • Complementary DNA codons specify amino acids with opposite hydropathies, supporting the observed interactions.

Conclusions:

  • The precise parallel or antiparallel alignment of peptides is crucial for establishing strong hydropathic complementarity.
  • A proposed model suggests hydrophilic residues orient towards the solvent, while hydrophobic residues form an interphase, driven by entropy for tight packing and strong interactions.