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Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay.

Cassia Maria Lins da Silva¹, Eliani Spinelli², Silvana Vianna Rodrigues¹

¹Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista S/N, Centro, Niterói, RJ 24020-150, Brazil.

|December 4, 2014

Summary

This study presents a rapid alkaline hydrolysis method for spectrophotometric collagen quantification. The new procedure is highly sensitive, requires minimal sample, and offers equivalent results to traditional methods.

Keywords:

Alkaline hydrolysis Collagen Hydroxyproline Protein Spectrophotometry

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Area of Science:

Analytical Chemistry
Biochemistry
Food Science

Background:

Accurate collagen quantification is crucial in various scientific fields.
Traditional methods like Association of Official Analytical Chemists (AOAC) acid hydrolysis are time-consuming and require large sample amounts.
Existing alkaline hydrolysis methods may lack sensitivity or require optimization.

Purpose of the Study:

To develop and optimize a preparative protein alkaline hydrolysis procedure for spectrophotometric collagen quantification.
To enhance the sensitivity and efficiency of collagen analysis.
To provide a method suitable for small, fresh solid or liquid samples.

Main Methods:

Optimization of NaOH concentration, hydrolysis time, buffer strength, and pH control.
Spectrophotometric analysis of collagen hydrolysates.
Comparison with the AOAC acid hydrolysis method using porcine liver and sausage samples.

Main Results:

The optimized alkaline hydrolysis procedure demonstrated a 5-fold increase in sensitivity compared to other alkaline methods.
Reaction time was reduced from 16 hours to 40 minutes.
Sample requirement decreased from 4 g to 3-20 mg, yielding equivalent results.

Conclusions:

The developed alkaline hydrolysis method is a sensitive, rapid, and efficient alternative for collagen quantification.
This procedure is suitable for analyzing small sample quantities in various matrices.
The method offers significant advantages over conventional acid hydrolysis techniques.