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Establishment and characterization of an Epstein-Barr virus transformed cell line with strong phagocytic activity
P von den Driesch1, R Bhardwaj, H D Flad
1Institut für Immunologie, Universität Münster, West Germany.
Abstract:
An immunoglobulin M (IgM)-positive cell line, Ms 28, apparently spontaneously transformed by Epstein-Barr virus (EBV) was established from peripheral blood cells of a patient with immature myeloblastic leukemia. It has been characterized according to phenotype, cytochemistry, and membrane antigen pattern. The cell line expresses lymphoid markers like CD 19, CD 22, and CD 30 and synthesizes and secretes IgM. Monocyte markers CD 11c, CD 14, and CD 15 are absent. Neither interleukin-1 (IL-1), nor tumor necrosis factor (TNF-alpha) are produced. But Ms 28 cells show strong phagocytic activity and engulf Latex particles and sheep RBCs (SRBCs) that need not to be opsonized. The phagocytic activity can be inhibited by chloroquine. Both phagocytosis and EBV nuclear-antigen (EBNA) expression can be observed in one and the same cell. Ms 28 cells might be useful to study immunologic activities like antigen processing and presentation.
Insights
A novel Epstein-Barr virus (EBV)-transformed cell line, Ms 28, exhibits both lymphoid markers and significant phagocytic activity. This unique cell line aids in studying antigen processing and presentation.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Established an immunoglobulin M (IgM)-positive cell line, Ms 28, from peripheral blood of a patient with immature myeloblastic leukemia.
- The cell line, Ms 28, was spontaneously transformed by Epstein-Barr virus (EBV).
Purpose of the Study:
- To characterize the phenotype, cytochemistry, and membrane antigen pattern of the Ms 28 cell line.
- To investigate the immunologic activities of the Ms 28 cell line, including phagocytosis and antigen presentation.
Main Methods:
- Phenotypic characterization using lymphoid markers (CD 19, CD 22, CD 30) and monocyte markers (CD 11c, CD 14, CD 15).
- Assessment of cytokine production (IL-1, TNF-alpha).
- Evaluation of phagocytic activity using Latex particles and sheep red blood cells (SRBCs), and inhibition studies with chloroquine.
- Detection of EBV nuclear antigen (EBNA) expression.
Main Results:
- Ms 28 cells express lymphoid markers and secrete IgM, lacking monocyte markers and cytokine production.
- Demonstrated strong, non-opsonin-dependent phagocytic activity inhibitable by chloroquine.
- Observed co-expression of phagocytosis and EBV nuclear-antigen (EBNA) within the same Ms 28 cells.
Conclusions:
- The Ms 28 cell line presents a unique combination of lymphoid characteristics and phagocytic function.
- This EBV-transformed cell line is a valuable tool for investigating cellular processes like antigen processing and presentation.