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Updated: Apr 20, 2026

Methods for the Self-integration of Megamolecular Biopolymers on the Drying Air-LC Interface
Published on: April 7, 2017
Separation properties of saccharides on a hydrophilic stationary phase having hydration layer formed zwitterionic
Waka Kamichatani1, Yoshinori Inoue2, Atsushi Yamamoto3
1Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi-shi, Tokyo 206-8577, Japan.
Abstract:
A novel water-holding adsorbent bonded with a zwitterionic polymer, diallylamine-maleic acid copolymer, was developed. With this adsorbent, hydrophilic solutes are partitioned by a hydration layer that forms on the zwitterions, as a main separating force. When the adsorbent was used to separate saccharides by normal-phase partition chromatography, the saccharides eluted in the order, mono-, di- and trisaccharide. The elution profile for mono- and di-saccharides was similar but not identical to that on anion exchange columns. This indicated that the adsorbent exhibited a complex retention behavior by the existence of both anion and cation exchange moieties in the functional polymer. Selecting Na(+) as a counter-ion of the maleate moiety enhanced the retention of saccharide. When used in an high performance liquid chromatography (HPLC) system with gradient elution, the adsorbent enabled the simultaneous analysis of mono-, di- and oligosaccharides.
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