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Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Mechanism of Angiogenesis

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Development of Blood Vessels

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The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
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Related Experiment Video

Updated: Apr 20, 2026

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

Published on: June 20, 2020

Vascular casting for the study of vascular morphogenesis.

Maximilian Ackermann¹, Moritz A Konerding

¹Department of Oral and Maxillofacial Surgery, Institute of Functional and Clinical Anatomy, University Medical Center Mainz, Mainz, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|December 4, 2014

Summary

Vascular corrosion casting visualizes blood vessel networks in tissues. This technique aids in studying microvascularity and angiogenesis in various conditions like tumor growth and regeneration.

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Area of Science:

Vascular biology and morphology analysis.

Background:

Microvascularity and angiogenesis are crucial in normal growth and diseases like inflammation, tumor growth, macular degeneration, and tissue regeneration.
Vascular corrosion casting is a key method for analyzing blood vessel morphology and architecture.

Purpose of the Study:

To detail the application of vascular corrosion casting for analyzing microvascular morphology and architecture.

Main Methods:

Vascular corrosion casting of organs and tissues (tumors, brains, embryos, chorioallantoic membrane).
Analysis of microvascular casts using scanning electron microscopy (SEM), micro computed tomography (μCT), and synchrotron radiation-based micro computed tomography (SRμCT).

Main Results:

Detailed two- and three-dimensional morphological and architectural evaluation of blood vessels.
Quantification of vascular structures through advanced imaging techniques.

Conclusions:

Vascular corrosion casting provides comprehensive insights into microvascular networks.
SEM, μCT, and SRμCT enable detailed visualization and quantification of vascular morphology for research and diagnostics.