Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Insulin Formulations: Types and Delivery01:27

Insulin Formulations: Types and Delivery

1.2K
Insulin preparations are categorized by their duration of action into short-acting and long-acting types. Two strategies are used to modify insulin's absorption and pharmacokinetic profile: slowing the absorption post-subcutaneous injection, or altering human insulin's amino acid sequence or protein structure. These changes retain the insulin's ability to bind to the insulin receptor, but alter its behavior in solution or after injection.
Short-acting insulins are divided into...
1.2K
Insulin: Biosynthesis, Chemistry, and Preparation01:25

Insulin: Biosynthesis, Chemistry, and Preparation

2.2K
The endoplasmic reticulum (ER) of pancreatic β-cells synthesizes preproinsulin, which consists of a signal peptide, A and B chains, and a C-peptide. Preproinsulin is then cleaved and folded into proinsulin, which translocates to the Golgi apparatus for sorting and packaging into secretory granules. In these granules, enzymatic clipping generates insulin and C-peptide.
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment...
2.2K
Insulin: Dosing Regimen and Adverse Effects01:16

Insulin: Dosing Regimen and Adverse Effects

1.3K
Insulin-replacement therapy usually includes both long-acting insulin (basal) and short-acting insulin (to cater to postprandial needs). In a diverse group of type 1 diabetes patients, the average daily insulin dose is typically 0.5-0.7 units/kg body weight. However, obese patients and pubertal adolescents may need more due to insulin resistance.
The basal dose constitutes about 40%-50% of the total daily dose, with the rest as premeal insulin. The mealtime insulin dose should mirror...
1.3K
Insulin: The Receptor and Signaling Pathways01:28

Insulin: The Receptor and Signaling Pathways

6.4K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
6.4K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

3.3K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
3.3K
Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

7.6K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Audibility-Based Informational Counseling in Pediatric Audiology Using the Speech Intelligibility Index: A Qualitative Study.

Ear and hearing·2026
Same author

A mixed-methods approach for understanding cancer center catchment area needs: An example of rural cancer disparities, needs, and resiliencies.

Cancer causes & control : CCC·2026
Same author

Efficacy of glucagon-like peptide-1 receptor analogues in kidney transplant recipients with diabetes: A single-centre retrospective study.

Diabetic medicine : a journal of the British Diabetic Association·2026
Same author

Addressing inequalities in access and care in type 1 diabetes - The North-East London Type 1 Diabetes Transformation (NATALIE) project.

Primary care diabetes·2026
Same author

Diabetes: A multisystem disease needing multidisciplinary care.

Clinical medicine (London, England)·2026
Same author

Using automated insulin delivery to address the clinical challenges of glycemic management in people with type 1 diabetes and kidney failure on maintenance hemodialysis.

Journal of diabetes investigation·2025

Related Experiment Video

Updated: Apr 20, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
07:30

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Published on: May 10, 2018

9.9K

Insulin for the uninitiated.

Tahseen A Chowdhury1, Roisin Wright2, Mary Charlton3

  • 1The Royal London Hospital, London, UK; Tahseen.Chowdhury@bartshealth.nhs.uk.

Clinical Medicine (London, England)
|December 4, 2014
PubMed
Summary
This summary is machine-generated.

Insulin therapy is crucial for diabetes management but often poorly monitored in hospitals. This guide helps non-specialist clinicians understand insulin use, improving patient care and safety.

Keywords:
Diabetesinsulin therapy

More Related Videos

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
05:52

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Published on: June 30, 2011

20.6K
An In Ovo Model for Testing Insulin-mimetic Compounds
06:09

An In Ovo Model for Testing Insulin-mimetic Compounds

Published on: April 23, 2018

11.3K

Related Experiment Videos

Last Updated: Apr 20, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
07:30

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Published on: May 10, 2018

9.9K
Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
05:52

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Published on: June 30, 2011

20.6K
An In Ovo Model for Testing Insulin-mimetic Compounds
06:09

An In Ovo Model for Testing Insulin-mimetic Compounds

Published on: April 23, 2018

11.3K

Area of Science:

  • Endocrinology
  • Diabetes Management
  • Internal Medicine

Background:

  • Insulin therapy is vital for many diabetic patients.
  • The UK National Diabetes Inpatient Audit (NaDIA) highlights poor inpatient insulin management.
  • Non-specialist clinicians require guidance on safe insulin use.

Purpose of the Study:

  • To demystify insulin therapy for non-specialist clinicians.
  • To provide essential knowledge on indications, types, and regimens of insulin.
  • To guide safe insulin prescribing and dose adjustment in hospital settings.

Main Methods:

  • Review of current guidelines and audit findings.
  • Synthesis of key information on insulin pharmacology and clinical practice.
  • Educational content aimed at healthcare professionals.

Main Results:

  • Identified critical knowledge gaps in non-specialist insulin management.
  • Outlined essential components of inpatient insulin therapy.
  • Emphasized the importance of specialist diabetes team support.

Conclusions:

  • Improved understanding and management of insulin therapy are needed in hospitals.
  • Non-specialist clinicians play a key role in safe insulin use.
  • Addressing these issues can enhance patient outcomes in diabetes care.