Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.4K
MicroRNAs01:22

MicroRNAs

25.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
25.1K
MicroRNAs01:22

MicroRNAs

12.1K
12.1K
Epigenetic Regulation01:46

Epigenetic Regulation

34.6K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.6K
Epigenetic Regulation01:37

Epigenetic Regulation

4.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.3K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

920
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
920

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circular RNAs: key regulators in cancer stem cell dynamics.

Molecular genetics and genomics : MGG·2026
Same author

Targeting RelB cancer associated protein by using bioactive compounds from naturally growing <i>Chamaedorea seifrizii</i>: in silico and in vitro validation.

In silico pharmacology·2026
Same author

m6A modification and its clinical applications in gynaecological cancer.

Apoptosis : an international journal on programmed cell death·2026
Same author

Molecular and bioinformatics analysis of long non-coding RNAs in cervical cancer.

Chromosoma·2025
Same author

Design of a Multi-Epitope Vaccine using β-barrel Outer Membrane Proteins Identified in Chlamydia trachomatis.

The Journal of membrane biology·2025
Same author

The human microbiome: redefining cancer pathogenesis and therapy.

Cancer cell international·2025

Related Experiment Video

Updated: Apr 20, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K

miRNA and methylation: a multifaceted liaison.

Ravindresh Chhabra1

  • 1Department of Biotechnology, Panjab University, Department of Biotechnology, Panjab University, Sector-14, Chandigarh 160014 (India). ravindresh@pu.ac.in, ravindreshchhabra@gmail.com.

Chembiochem : a European Journal of Chemical Biology
|December 4, 2014
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) and DNA methylation are key gene regulators. This review explores their complex interplay, including how DNA and mRNA methylation affects miRNA function and vice versa.

Keywords:
DNA methylationDNMTepigeneticsgenomicsm5Cm6A

More Related Videos

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.3K
Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

14.1K

Related Experiment Videos

Last Updated: Apr 20, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.3K
Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

14.1K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) and DNA methylation are crucial regulators of gene expression.
  • Aberrations in miRNA expression or DNA methylation contribute to various pathological conditions.
  • DNA methylation can suppress miRNA transcription by targeting promoter CpG islands.

Purpose of the Study:

  • To review the intricate relationship between microRNA and methylation at both DNA and mRNA levels.
  • To highlight the reciprocal regulatory mechanisms between miRNAs and DNA methyltransferases.
  • To explore the impact of mRNA methylation on miRNA-mRNA interactions.

Main Methods:

  • Literature review of studies on miRNA and DNA methylation.
  • Analysis of research on mRNA methylation (m6A and m5C) in 3'-untranslated regions.
  • Synthesis of findings on the interplay between epigenetic modifications and miRNA activity.

Main Results:

  • DNA methylation inhibits miRNA transcription, while miRNAs can target DNA methyltransferases.
  • mRNA methylation, specifically m6A and m5C, occurs in 3'-untranslated regions, affecting mRNA stability.
  • The influence of mRNA methylation on miRNA binding and function remains an underexplored area.

Conclusions:

  • The interaction between miRNAs and methylation is complex, involving DNA and mRNA modifications.
  • Understanding these epigenetic regulatory networks is vital for deciphering pathological mechanisms.
  • Further research is needed to elucidate the functional consequences of mRNA methylation on miRNA activity.