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Quantitative trace analysis of complex mixtures using SABRE hyperpolarization.

Nan Eshuis1, Bram J A van Weerdenburg, Martin C Feiters

  • 1Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen (The Netherlands).

Angewandte Chemie (International Ed. in English)
|December 4, 2014
PubMed
Summary
This summary is machine-generated.

Signal amplification by reversible exchange (SABRE) now enables quantitative analysis of complex mixtures. This nuclear spin hyperpolarization technique allows low-micromolar concentration determination from single scans, overcoming previous efficiency variations.

Keywords:
NMR spectroscopySABREanalytical chemistryhyperpolarizationtrace analysis

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Area of Science:

  • Analytical Chemistry
  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Hyperpolarization Techniques

Background:

  • Signal amplification by reversible exchange (SABRE) is a novel hyperpolarization method enhancing NMR signals of small molecules.
  • Previous applications of SABRE have not focused on quantitative analysis due to variable efficiency across different molecules and nuclei.
  • The quantitative potential of SABRE for complex mixtures remained unexplored.

Purpose of the Study:

  • To demonstrate the first application of SABRE for quantitative analysis of a complex mixture.
  • To establish a method for determining low-micromolar analyte concentrations using SABRE.
  • To overcome the challenge of variable SABRE efficiency in quantitative measurements.

Main Methods:

  • Utilized signal amplification by reversible exchange (SABRE) for NMR signal enhancement.
  • Applied a standard-addition approach for quantitative determination of analytes.
  • Acquired single-scan SABRE spectra for analysis.

Main Results:

  • Successfully quantified analytes at low micromolar concentrations in a complex mixture.
  • Demonstrated the feasibility of quantitative analysis despite thousands of competing binding equilibria.
  • Overcame the inherent variability of SABRE efficiency for accurate concentration determination.

Conclusions:

  • SABRE can be effectively employed for quantitative analysis of complex mixtures.
  • The standard-addition approach is suitable for accurate quantification using single-scan SABRE spectra.
  • This work expands the utility of SABRE beyond signal enhancement to precise concentration measurements.