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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Updated: Apr 20, 2026

Author Spotlight: Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
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Far-red fluorogenic probes for esterase and lipase detection.

Katie R Tallman1, Kimberly E Beatty

  • 1Department of Physiology and Pharmacology, Department of Biomedical Engineering, Oregon Health & Science University, 2730 SW Moody Avenue, CL3B, Portland, OR 97201 (USA).

Chembiochem : a European Journal of Chemical Biology
|December 4, 2014
PubMed
Summary

New fluorogenic esterase probes were developed using a far-red fluorophore. These probes offer sensitive, real-time detection of enzyme activity, particularly in bacteria like Mycobacterium tuberculosis.

Keywords:
esterasesfluorescencefluorogenic probeslipasestuberculosis

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Microbiology

Background:

  • Fluorogenic enzyme probes enable sensitive, real-time detection of enzyme activity.
  • These probes are valuable for studying bacterial enzymes, including those in Mycobacterium tuberculosis.

Purpose of the Study:

  • To develop novel fluorogenic esterase probes with enhanced optical properties.
  • To utilize these probes for detecting esterase activity in mycobacterial lysates.

Main Methods:

  • Synthesis of two fluorogenic esterase probes derived from 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO).
  • Validation of probes using commercial enzymes and comparison with existing substrates.
  • Application of probes to analyze esterase activity in protein gel-resolved Mycobacterium tuberculosis lysates.

Main Results:

  • The new DDAO-derived probes exhibit hydrolysis-dependent fluorescence with excitation above 600 nm and a quantum yield of 0.40.
  • Probes successfully detected esterase activity in mycobacterial lysates, demonstrating their utility.
  • Performance was validated against known resorufin- and fluorescein-based esterase substrates.

Conclusions:

  • The developed fluorogenic esterase probes offer improved optical properties for enzyme detection.
  • These novel tools are effective for characterizing esterase activity in complex biological samples like mycobacterial lysates.
  • The probes are suitable for diverse applications requiring sensitive esterase detection.