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Oncogene-related growth factors and growth factor receptors in human malignant glioma-derived cell lines

G R Harsh1, M L Rosenblum, L T Williams

  • 1Department of Neurological Surgery, School of Medicine, University of California, San Francisco.

Journal of Neuro-Oncology
|May 1, 1989
PubMed

Insights

Malignant glioma cells secrete growth factors that stimulate proliferation. These cells possess receptors for platelet-derived growth factor (PDGF) and epidermal growth factor (EGF), suggesting a mechanism for uncontrolled tumor growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Oncogenes drive malignant cell transformation.
  • Specific oncogenes (v-sis, v-erb B) relate to growth factors (PDGF) and their receptors (EGF receptor).
  • Malignant gliomas may proliferate due to autocrine stimulation by secreted mitogenic factors.

Purpose of the Study:

  • Investigate if malignant glioma cells produce mitogenic factors.
  • Determine if these cells express receptors for such factors.
  • Explore the role of growth factor signaling in glioma proliferation.

Main Methods:

  • Culturing of human malignant glioma cell lines.
  • Assessing DNA synthesis stimulation in response to secreted factors.
  • Characterizing secreted factors using binding and autophosphorylation assays for PDGF and EGF receptors.

Main Results:

  • All tested glioma cell lines secreted factors stimulating DNA synthesis.
  • One secreted factor resembled PDGF.
  • Multiple cell lines expressed functional PDGF and EGF receptors.
  • One cell line showed extremely high EGF receptor levels, potentially linked to erb B oncogene and chromosome 7 abnormalities.

Conclusions:

  • Malignant gliomas can produce autocrine mitogenic factors.
  • Glioma cells express functional PDGF and EGF receptors.
  • Aberrant growth factor signaling, particularly involving EGF receptors, may contribute to glioma pathogenesis.

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