Related Experiment Video

Updated: Apr 20, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

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TSPO interacts with VDAC1 and triggers a ROS-mediated inhibition of mitochondrial quality control

Jemma Gatliff1, Daniel East, James Crosby

  • 1a Department of Comparative Biomedical Sciences; The Royal Veterinary College ; University of London ; London , UK.

Autophagy
|December 4, 2014
PubMed

Keywords:
ATP5B, ATP synthase, H+ transporting, mitochondrial F1 complex, β subunitDAPI, 4’, 6-diamidino-2-phenylindoleDHE, dihydroethidiumDNM1L, dynamin 1-likeFCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazoneGAPDH, glyceraldehyde-3-phosphate dehydrogenaseGSH, glutathioneMAP1LC3/LC3, microtubule-associated protein 1 light chain 3MCB, monochlorobimaneMEFs, mouse embryonic fibroblastsMnTBAP, manganese [III] tetrakis (4-benzoic acid) porphyrinMβCD, methyl-β-cyclodextrinNRF1, nuclear respiratory factor 1OMM, outer mitochondrial membranePARK2PBS, phosphate-buffered salinePINK1, PTEN-induced putative kinase 1PRKCE, protein kinase C, epsilonRM, recording mediumROSROS, reactive oxygen speciesRT, room temperatureSQSTM1, sequestosome 1TFAM, transcription factor A, mitochondrialTMRM, tetramethylrhodamine methyl esterTSPOTSPO, translocator proteinVDAC1, voltage-dependent anion channel 1YFP, yellow fluorescent proteinmitochondriamitophagymtRFP, mitochondrially targeted red fluorescent proteinnsc, nonsilencing controlsiRNA, small interfering ribonucleic acidubiquitinΔψm, mitochondrial membrane potential

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