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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Gut microbiota and metabolic syndrome.
Davide Festi1, Ramona Schiumerini1, Leonardo Henry Eusebi1
1Davide Festi, Ramona Schiumerini, Leonardo Henry Eusebi, Giovanni Marasco, Martina Taddia, Antonio Colecchia, Department of Digestive Diseases and Internal Medicine, University of Bologna, S Orsola Hospital, 40138 Bologna, Italy.
World Journal of Gastroenterology
|December 5, 2014
Summary
The gut microbiota plays a key role in metabolic syndrome development and is influenced by diet. Manipulating gut microbes with prebiotics or probiotics may improve metabolic health and aid weight loss, but more research is needed.
Area of Science:
- Microbiology
- Metabolic Health
- Dietary Science
Background:
- The gut microbiota significantly influences metabolic syndrome pathogenesis in humans and animal models.
- Diet profoundly impacts gut microbial composition and function, affecting host metabolic balance.
- Imbalances in gut microbiota can lead to metabolic endotoxemia, systemic inflammation, and insulin resistance.
Purpose of the Study:
- To review the role of gut microbiota in metabolic syndrome.
- To explore how diet, weight loss interventions, and bariatric surgery alter gut microbiota.
- To assess the potential of microbiota manipulation (prebiotics, probiotics) for metabolic health.
Main Methods:
- Literature review of studies on gut microbiota, metabolic syndrome, diet, and therapeutic interventions.
- Analysis of mechanisms by which gut microbiota affects host metabolism and inflammation.
- Evaluation of evidence for prebiotics and probiotics in managing metabolic dysfunction.
Main Results:
- Gut microbiota composition is altered by diet, weight loss, and bariatric surgery, influencing treatment outcomes.
- Gut microbial dysbiosis can contribute to metabolic endotoxemia, inflammation, and insulin resistance.
- Prebiotics and probiotics show potential in reducing inflammation and improving gut barrier function.
Conclusions:
- Gut microbiota is a critical factor in metabolic syndrome.
- Dietary interventions and bariatric surgery impact gut microbiota, affecting therapeutic success.
- Further research is required to establish the clinical efficacy of prebiotics and probiotics for metabolic health.
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