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Updated: Apr 20, 2026

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
Salmonella contamination risk points in broiler carcasses during slaughter line processing
Walter Rivera-Pérez1, Elías Barquero-Calvo2, Rebeca Zamora-Sanabria3
1Veterinary Medicine School, National University, 40206 Heredia, Costa Rica; Animal Science School, University of Costa Rica, 11501 San José, Costa Rica.
Salmonella contamination is a significant risk in poultry processing. Evisceration and spray washing steps pose the highest risk for cross-contamination, potentially leading to hundreds of salmonellosis cases annually.
Area of Science:
- Food Safety
- Microbiology
- Public Health
Background:
- Salmonella is a primary foodborne pathogen linked to poultry.
- Poultry processing plants require rigorous contamination control measures.
Purpose of the Study:
- Identify critical Salmonella contamination points in a Costa Rican chicken processing plant.
- Analyze the risk of salmonellosis from contaminated poultry consumption.
Main Methods:
- Qualitative and quantitative Salmonella testing (CFU counts) on carcasses.
- Risk analysis based on contamination prevalence and consumption data.
Main Results:
- Salmonella detected in 26% of carcasses; 60% of flocks positive.
- Highest Salmonella counts post-bleeding (6.1 log CFU/carcass).
- Evisceration and spray washing increased contamination (10-40%) and counts (3.9-5.1 log CFU/carcass).
Conclusions:
- Evisceration and spray washing are key risk points for Salmonella cross-contamination.
- An estimated 272 annual salmonellosis cases result from contaminated chicken consumption.
- Targeted interventions during evisceration and washing are crucial for reducing risk.
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