Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.4K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acoustophoresis of monodisperse oil droplets in water: Effect of symmetry breaking and non-resonance operation on oil trapping behavior.

Biomicrofluidics·2024
Same author

Micromolar Metabolite Measurement in an Electronically Multiplexed Format.

IEEE transactions on bio-medical engineering·2022
Same author

Is human platelet lysate (hPL) the ideal candidate to substitute the foetal bovine serum for cell-therapy translational research?

Journal of translational medicine·2021
Same author

Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review.

Journal of translational medicine·2020
Same author

Anatomically accurate 3D modelling and printing in a case of obstetric brachial plexus injury.

JPRAS open·2020
Same author

Negative Refraction in Time-Varying Strongly Coupled Plasmonic-Antenna-Epsilon-Near-Zero Systems.

Physical review letters·2020

Related Experiment Video

Updated: Apr 19, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.5K

Dynamic acoustic field activated cell separation (DAFACS).

G D Skotis1, D R S Cumming, J N Roberts

  • 1School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK. Anne.Bernassau@glasgow.ac.uk.

Lab on a Chip
|December 5, 2014
PubMed
Summary
This summary is machine-generated.

A novel dynamic acoustic field method enables label-free cell separation for regenerative medicine. This technique achieves high purity and efficiency for diverse cell types, offering a safe and biocompatible solution.

More Related Videos

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Published on: May 14, 2020

5.7K
Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
06:51

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

Published on: August 21, 2018

7.6K

Related Experiment Videos

Last Updated: Apr 19, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

14.5K
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
07:06

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Published on: May 14, 2020

5.7K
Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations
06:51

Microparticle Manipulation by Standing Surface Acoustic Waves with Dual-frequency Excitations

Published on: August 21, 2018

7.6K

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Acoustic Separation

Background:

  • Cell and particle separation technologies are advancing, driven by diagnostics and stem cell research.
  • Acoustic micro-particle separation is a promising label-free, high-throughput method for regenerative medicine.

Purpose of the Study:

  • To demonstrate a novel dynamic acoustic field for separating cells of arbitrary sizes.
  • To showcase the method's effectiveness for particles of varying diameters and densities.
  • To apply the technique for isolating dorsal root ganglion cells.

Main Methods:

  • Utilizing a dynamic acoustic field for particle and cell manipulation.
  • Testing separation of particles with diameters from 6 to 45 micrometers.
  • Evaluating separation of particles with different densities in a heterogeneous medium.

Main Results:

  • Successful separation of particles based on size and density.
  • Demonstrated isolation of dorsal root ganglion cells.
  • Achieved high purity (up to 100%) and efficiency (up to 100%) in cell separation.

Conclusions:

  • The dynamic acoustic field offers a shearless, label-free, and low-damage method for cell separation.
  • This technique is safe, biocompatible, and suitable for biological applications.
  • The method demonstrates significant potential for high-throughput cell isolation in regenerative medicine.