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Updated: Apr 19, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Physiological and molecular functions of the cytosolic peptide:N-glycanase
Hiroto Hirayama1, Akira Hosomi1, Tadashi Suzuki1
1Glycometabolome Team, System Glycobiology Research Group, RIKEN-Max Planck Joint Center for System Chemical Biology, Global Research Cluster, RIKEN, Hirosawa 2-1, Japan.
Abstract:
Peptide:N-glycanase (PNGase) is a deglycosylating enzyme that acts on N-glycoproteins. A growing evidence exists to indicate that the cytosolic form of PNGase, which is ubiquitously distributed throughout eukaryotes, is not only implicated in the efficient degradation of misfolded glycoproteins destined for the proteasomal degradation but also in the generation of free oligosaccharides as the initial step in the non-lysosomal catabolism of N-glycans. This article summarizes the current state of our knowledge of the physiological and molecular functions of the cytosolic PNGase in a model organism, Saccharomyces cerevisiae, and also discusses the functional/structural diversities of this molecule within eukaryotes.
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