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Published on: January 7, 2019
[Expression of microRNAs in lung homogenates in rats with chronic obstructive pulmonary disease]
1Department of Emergency, PLA General Hospital, Beijing 100853, China, Corresponding author: Li Tanshi,
Objective:
To determine the expression spectrum of microRNA (miRNA) in the lung homogenate of rats with chronic obstructive pulmonary disease(COPD) or healthy rats, to look for differentially expressed miRNA, and to explore their potential role in pathogenesis of COPD.
Methods:
Twenty male Sprague-Dawley (SD) rats were randomly divided into normal control group and COPD model group, with 10 rats in each group. COPD rat models were reproduced by smoke inhalation as well as intratracheal instillation of lipopolysaccharide (LPS). The samples of the lung were harvested, and the histopathological examination of the right lung was carried out to evaluate the degree of lung injury. Total RNAs were isolated from the left lung. The miRNA expressions in lung tissue of rats with COPD or normal rats were determined by miRNA chip eichnology to screen the miRNA with differential expression, and hierarchical clustering analysis was made. The data were analyzed to study the expression difference of miRNAs between the two groups, and to construct the miRNA-Target network.
Results:
Compared with normal control group, 20 miRNA with increased expression were found in COPD model group, the top 10 were miR-30c-2, miR-199a-5p, miR-30a, miR-145, miR-151, miR-674-5p, miR-214, miR-423, miR-28 and miR-181b, and only miR-376b-3p showed down-regulation. Hierarchical clustering analysis showed that significant differences in individual miRNA in lung tissue between rats of two groups were found. However, similar samples could not be gathered well, thus it may give rise to inconsistency during the sample preparation. Many miRNAs had multiple target genes in miRNA-Target network, such as miR-30c-2, miR-145, miR-181b, miR-181a, miR-181d, miR-199.
Conclusions:
The study reveals that there is a variance of miRNAs expression profile in lung homogenates between COPD group and the normal control group of rats, and many miRNAs have multiple target genes, such as miR-30c-2, miR-145, miR-181b, miR-181a, miR-181d, and miR-199. Therefore, the results may be valuable to centain extent for the interpretation of COPD pathogenesis.
Insights
This study found significant differences in microRNA (miRNA) expression in rat lungs with chronic obstructive pulmonary disease (COPD). These findings offer insights into the molecular mechanisms underlying COPD development.
Area of Science:
- Pulmonary Medicine
- Molecular Biology
- Genetics
Context:
- Chronic obstructive pulmonary disease (COPD) is a major global health concern.
- Understanding the molecular mechanisms of COPD pathogenesis is crucial for developing effective treatments.
- MicroRNAs (miRNAs) are small non-coding RNAs that play significant roles in gene regulation and have been implicated in various diseases.
Purpose:
- To investigate the differential expression of miRNAs in the lung homogenate of rats with experimentally induced COPD compared to healthy controls.
- To identify specific miRNAs that are dysregulated in COPD.
- To explore the potential role of these differentially expressed miRNAs in the pathogenesis of COPD.
Summary:
- COPD rat models were established using smoke inhalation and lipopolysaccharide (LPS) instillation.
- miRNA expression profiling revealed 20 upregulated miRNAs and 1 downregulated miRNA (miR-376b-3p) in the COPD group.
- Hierarchical clustering showed distinct miRNA expression patterns between COPD and control groups, with several miRNAs like miR-30c-2 and miR-145 exhibiting multiple target genes.
Impact:
- The study identified a unique miRNA expression profile in COPD lungs, suggesting miRNAs as potential biomarkers for COPD diagnosis and progression.
- The findings contribute to a better understanding of the molecular underpinnings of COPD pathogenesis.
- The identified miRNAs and their targets may represent novel therapeutic targets for COPD treatment.
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