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Published on: March 21, 2018
A carboxylated graphene and aptamer nanocomposite-based aptasensor for sensitive and specific detection of hemin
Ling Gao1, Yinghong Xiao1, Yanping Wang1
1Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
Abstract:
A unique nanocomposite was crafted by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR). Infrared spectroscopy, Raman spectroscopy and diffuse reflectance spectra suggested that -NHCO- covalent bonds were formed between HBA and COO-GR. The resulting COO-GR/HBA functionalized electrode was used as a novel label-free biosensor. The square wave voltammetry was employed to realize the selective and specific detection of hemin. The obtained aptasensor possessed excellent performance with a detection limit of 0.64 nmol L(-1) (S/N=3) and a linear range from 1 to 150 nmol L(-1). Moreover, COO-GR was shown to be a promising candidate in making aptasensors, carrying advantages over graphene in terms of the simplicity of sensor preparation and the reduction of background noise.
