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Related Experiment Videos

Unusual precipitins in pathological human sera.

D S Kim1, R Anthone, S Anthone

  • 1Department of Microbiology, School of Medicine, State University of New York, Buffalo.

International Archives of Allergy and Applied Immunology
|January 1, 1989
PubMed
Summary

Unexpected precipitation reactions were observed between renal transplant recipient serum and pathological human sera. These reactions, involving IgM antibodies and a beta-globulin precipitinogen, occurred frequently in infectious mononucleosis and other immune-related conditions.

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Area of Science:

  • Immunology
  • Transplantation Medicine
  • Clinical Chemistry

Background:

  • Double diffusion gel precipitation tests are standard immunological assays.
  • Renal graft recipients may develop unique serum components.
  • Pathological human sera can contain various autoantibodies and immune complexes.

Purpose of the Study:

  • To investigate unexpected precipitation reactions observed between a renal graft recipient's serum and pathological human sera.
  • To identify the nature of the precipitin and precipitinogen involved in these reactions.
  • To determine the frequency of these reactions in different pathological conditions.

Main Methods:

  • Double diffusion gel precipitation tests were performed.
  • Sera from renal graft recipients and patients with various pathologies were analyzed.

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  • Immunochemical characterization of the precipitin and precipitinogen was conducted, including thermostability and electrophoretic mobility analysis.

    • Main Results:

    • Strong precipitation reactions were detected between a renal graft recipient's serum and multiple pathological human sera.
    • Positive reactions were most frequent in patients with infectious mononucleosis (83%), chronic Epstein-Barr virus infections (72%), rheumatoid arthritis (57%), lepromatous leprosy (57%), and AIDS (44%).
    • The precipitin was identified as an IgM antibody, and the precipitinogen was a thermostable beta-globulin component in the transplantation serum.

    Conclusions:

    • The study identified significant precipitation reactions between a renal transplant recipient's serum and sera from patients with specific infectious and autoimmune diseases.
    • The observed reactions involve IgM antibodies and a thermostable beta-globulin.
    • The underlying cause and clinical significance of these reactions remain unexplained.