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Updated: Apr 19, 2026
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
F L E Spositto1, P A Z Campanerut2, L D Ghiraldi2
1Programa de Pós Graduação em Biociências e Fisiopatologia Universidade Estadual de Maringá MaringáPR Brazil Programa de Pós Graduação em Biociências e Fisiopatologia, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Abstract:
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
