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Published on: October 24, 2016
Production of bioethanol using agricultural waste: banana pseudo stem
Snehal Ingale1, Sanket J Joshi2, Akshaya Gupte3
1Department of Microbiology Natubhai V. Patel College of Pure and Applied Sciences Vallabh-VidyanagarGujarat India Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh-Vidyanagar, Gujarat, India. ; Department of Microbiology ARIBAS New Vallabh Vidyanagar Gujarat India Department of Microbiology, ARIBAS New Vallabh Vidyanagar, Gujarat, India.
Abstract:
India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g%) gave maximum ethanol (17.1 g/L) with yield (84%) and productivity (0.024 g%/h) after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.
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