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Updated: Apr 19, 2026
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
Published on: March 12, 2015
Quenched electrochemiluminescence of Ag nanoparticles functionalized g-C₃N₄ by ferrocene for highly sensitive
Xiaojian Li1, Zhankui Guo1, Jianxiu Li1
1Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Abstract:
In this paper, a novel sandwich electrochemiluminescence (ECL) immunosensor was constructed by ferrocene for quenching Ag nanoparticles functionalized g-C3N4 (Ag@g-C3N4) emission. The prepared Ag@g-C3N4 had strong and stable ECL signals compared to pure g-C3N4 and primary antibody (Ab1) can be immobilized on Ag@g-C3N4 by adsorption of Ag nanoparticles. Ferrocene carboxylic acid (Fc-COOH) labeled secondary antibody was immobilized on Au doped mesoporous Al2O3 nanorods (Au@Al2O3-Fc-COOH@Ab2) as labels through adsorption ability of Au toward proteins. After a sandwich-type immunoreaction, a remarkable decrease of ECL signal was observed due to the ECL quenching of Ag@g-C3N4 by Au@Al2O3-Fc-COOH@Ab2. As a result, the change of ECL intensity has a direct relationship with the logarithm of CEA concentrations in the range of 1 pg mL(-1)-100 ng mL(-1) with a detection limit of 0.35 pg mL(-1) (S/N=3). Additionally, the proposed immunosensor shows high specificity, good reproducibility, and long-term stability.
