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Genetic identification of endogenous polytropic proviruses by using recombinant inbred mice
W N Frankel1, J P Stoye, B A Taylor
1Department of Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Abstract:
Forty-seven endogenous polytropic murine viruses (Pmv) were identified by examination of proviral-cellular DNA junction fragment segregation in recombinant inbred (RI) mice. Most Pmv loci were found in more than one of the seven RI progenitor strains analyzed, but only four were present in all strains. Chromosomal assignments for 41 Pmv loci were determined by comparing their RI strain distribution patterns with those of known genetic markers. Pmv loci were found dispersed throughout the genome, with chromosomes 1, 3, 4, 5, 7, 11, 12, 15, and 16 each carrying three or more proviruses. Linkage analysis in the AKXD RI set suggested that the gene encoding mink cell focus-forming virus resistance (Rcmfr) of DBA/2J mice is probably not a Pmv provirus. It was also deduced that no single, AKR/J-specific Pmv provirus is required as an env gene donor for thymomagenic mink cell focus-forming viruses. In addition, a Pmv provirus was very closely associated with the albino mutation on chromosome 7.
Insights
Researchers identified 47 endogenous polytropic murine viruses (Pmv) in mice, mapping their locations across the genome. This study provides a comprehensive catalog of Pmv loci and their distribution in mouse strains.
Area of Science:
- Genetics
- Virology
- Immunology
Background:
- Endogenous polytropic murine viruses (Pmv) are retroviruses integrated into the host genome.
- Understanding the distribution and genetic linkage of Pmv loci is crucial for studying their role in host genetics and disease.
Purpose of the Study:
- To identify and characterize endogenous polytropic murine viruses (Pmv) in recombinant inbred (RI) mice.
- To determine the chromosomal locations and genetic associations of Pmv loci.
Main Methods:
- Analysis of proviral-cellular DNA junction fragment segregation in seven RI mouse progenitor strains.
- Comparison of Pmv locus distribution patterns with known genetic markers for chromosomal assignment.
- Linkage analysis within the AKXD RI mouse set.
Main Results:
- Forty-seven endogenous polytropic murine viruses (Pmv) were identified.
- Pmv loci are dispersed throughout the mouse genome, with specific chromosomes showing higher proviral loads.
- A Pmv provirus was found to be closely associated with the albino mutation on chromosome 7.
- Evidence suggests Rcmfr gene is not a Pmv provirus and no single AKR/J-specific Pmv is essential for thymomagenic viruses.
Conclusions:
- The study provides a detailed map of endogenous polytropic murine virus (Pmv) loci across the mouse genome.
- The findings contribute to understanding the genetic basis of retroviral integration and host-virus interactions.
- Specific Pmv associations with host genes and mutations were identified, opening avenues for further research.