Related Experiment Video
Updated: Apr 19, 2026
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
Published on: April 5, 2024
Hyaluronic acid effect on adipose-derived stem cells. Biological in vitro evaluation
A Moreno1, A Martínez2, S Olmedillas2
1Servicio de Cirugía Ortopédica y Traumatología, Unidad de Rodilla, Hospital Universitario La Paz, Madrid, España.
Objectives:
To evaluate the in vitro effects of hyaluronic acid (HA) on adipose-derived stem cells (ASC) in order to consider the possibility of their combined used in the treatment of knee arthrosis.
Material And Methods:
The ASC cells were grown both in the presence and absence of AH, and several studies were carried out: proliferation (WST8) and cell viability studies (Alamar Blue® and Trypan Blue), possible chondrogenic differentiation (collagen type 2 expression) by RT-PCR, AH receptor expression (CD44) by flow cytometry and RT-QPCR, and expression of inflammatory and anti-inflammatory factors (IL-6, TGFß, IL-10) by RT-QPCR.
Results:
The number of ASC significantly increased after 7 days with HA (158±39%, p <0.05). Additionally, the cell viability of the ASC treated with HA after 1, 3, 5 and 7 days was similar to that of the control cells, being considered non-toxic. There were no changes observed in the expression of CD44 and chondrogenic differentiation. TGFß expression was not modified after AH treatment, but there was a 4-fold decrease in IL-6 expression and IL-10 expression increased up to 2-fold compared to control cells.
Conclusions:
Hyaluronic acid favours ASC proliferation without causing cellular toxicity, and inducing an anti-inflammatory profile in these cells. Hyaluronic acid appears to be a suitable vehicle for the intra-articular administration of mesenchymal stem cells.
