Novel magnetic cross-linked lipase aggregates for improving the resolution of (R, S)-2-octanol
Ying Liu1, Chen Guo, Chun-Zhao Liu
1National Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China.
Abstract:
Novel magnetic cross-linked lipase aggregates were fabricated by immobilizing the cross-linked lipase aggregates onto magnetic particles with a high number of -NH2 terminal groups using p-benzoquinone as the cross-linking agent. At the optimal fabrication conditions, 100% of immobilization efficiency and 139% of activity recovery of the magnetic cross-linked lipase aggregates were achieved. The magnetic cross-linked lipase aggregates were able to efficiently resolve (R, S)-2-octanol, and retained 100% activity and 100% enantioselectivity after 10 cycles of reuse, whereas the cross-linked lipase aggregates only retained about 50% activity and 70% enantioselectivity due to insufficient cross-linking. These results provide a great potential for industrial applications of the magnetic cross-linked lipase aggregates.
