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A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Published on: September 10, 2021

Tools for metabolic engineering in Streptomyces.

Valerie Bekker¹, Amanda Dodd, Dean Brady

¹a School of Molecular and Cell Biology; University of the Witwatersrand; Johannesburg, South Africa.

|December 9, 2014

Summary

Streptomyces bacteria are vital for producing beneficial secondary metabolites. This review details metabolic engineering tools developed over the last decade to enhance their production for pharmaceutical applications.

Keywords:

Streptomycetes genome scale metabolic models industrial production metabolic engineering secondary metabolites

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Area of Science:

Microbiology
Biotechnology
Metabolic Engineering

Background:

Streptomyces bacteria are crucial producers of valuable secondary metabolites.
These metabolites are of significant interest to academic and pharmaceutical sectors.
Extensive tools for metabolic engineering of Streptomyces have been developed.

Purpose of the Study:

To review and discuss metabolic engineering tools for Streptomyces.
Focus on literature from the past decade.
Highlight strategies for enhancing secondary metabolite production.

Main Methods:

Precursor engineering
Structural and regulatory gene engineering
Gene upregulation/downregulation
Genome shuffling
Genome-scale metabolic modeling

Main Results:

A wide array of metabolic engineering tools are available for Streptomyces.
These tools enable manipulation of biosynthetic gene clusters.
Genome-scale models aid in predicting and rationalizing engineering strategies.

Conclusions:

Metabolic engineering tools for Streptomyces are continuously advancing.
These developments simplify strategies for optimizing secondary metabolite yields.
Streptomyces remain a vital target for biotechnological applications.