Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

82.1K
Overview
82.1K
CRISPR01:59

CRISPR

60.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
60.4K
Transgenic Organisms00:53

Transgenic Organisms

6.7K
6.7K
Transgenic Organisms00:53

Transgenic Organisms

34.7K
Overview
34.7K
In vitro Mutagenesis01:16

In vitro Mutagenesis

5.7K
5.7K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.7K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-region sampling of the human small intestine using an ingestible device.

medRxiv : the preprint server for health sciences·2026
Same author

CUPID-seq enables highly multiplexed amplicon sequencing via combinatorial in-line dual indexing.

bioRxiv : the preprint server for biology·2026
Same author

VIBRANT: A phase 1 randomized trial of multi-strain vaginal L. crispatus live biotherapeutic products in people with bacterial vaginosis.

Cell host & microbe·2026
Same author

Correction: Distinct Distal Gut Microbiome Diversity and Composition in Healthy Children from Bangladesh and the United States.

PloS one·2026
Same author

VIRGO2: an enhanced gene catalog of the vaginal microbiome provides insights into its functional and ecology complexity.

Nature communications·2025
Same author

Toward a framework for risk mitigation of potential misuse of artificial intelligence in biomedical research.

Nature machine intelligence·2025

Related Experiment Video

Updated: Apr 19, 2026

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
09:39

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

10.4K

Gain-of-function experiments: time for a real debate.

W Paul Duprex1, Ron A M Fouchier2, Michael J Imperiale3

  • 1Boston University School of Medicine and the National Emerging Infectious Diseases Laboratories (NEIDL), Boston, Massachusetts 02118, USA.

Nature Reviews. Microbiology
|December 9, 2014
PubMed
Summary
This summary is machine-generated.

Dual use research of concern (DURC) involves life sciences research with potential for harm. Gain-of-function (GOF) experiments on potential pandemic pathogens (PPPs) spark debate on balancing benefits against biosafety and biosecurity risks.

More Related Videos

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

14.5K
In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

21.6K

Related Experiment Videos

Last Updated: Apr 19, 2026

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
09:39

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

10.4K
In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

14.5K
In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

21.6K

Area of Science:

  • Life Sciences
  • Biosecurity
  • Public Health

Background:

  • Dual use research of concern (DURC) is defined by the WHO as life sciences research with beneficial intent but potential for misuse.
  • Recent influenza virus studies have intensified discussions on DURC.
  • Gain-of-function (GOF) experiments, particularly on potential pandemic pathogens (PPPs), are central to this debate.

Conclusions:

  • The DURC debate necessitates careful consideration of scientific advancement versus global security.
  • Moving forward requires a balanced approach, engaging scientists, policymakers, and the public.
  • Establishing clear guidelines for GOF research is crucial for responsible scientific progress.