Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

3.5K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
3.5K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

4.3K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
4.3K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.8K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.8K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.8K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.8K
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

3.6K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.6K
Anchoring Junctions01:03

Anchoring Junctions

5.7K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.

Protein science : a publication of the Protein Society·2026
Same author

<i>Limousia</i> bacteria encode mucinolysome for mucin utilization in animal gut microbiomes.

Gut microbes·2026
Same author

Deconstruction by <i>C. thermocellum</i>-from microbe mediated to dynamic redistribution of cellulosomes.

Life science alliance·2026
Same author

Spatial constraints drive amylosome-mediated resistant starch degradation by Ruminococcus bromii in the human colon.

Nature communications·2025
Same author

Mucinolysome in gut microbiomes of farm animals and humans.

bioRxiv : the preprint server for biology·2025
Same author

Development of a thermophilic l-arabinose-inducible system in Acetivibrio thermocellus (Clostridium thermocellum).

Metabolic engineering·2025
Same journal

Unlocking the capacity of Mn-based Prussian blue cathodes in capacitive deionization.

Nature communications·2026
Same journal

Scaling biodiversity-stability relationships from populations to meta-communities across trophic levels.

Nature communications·2026
Same journal

Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.

Nature communications·2026
Same journal

Engineering all-organic electrocatalysts with asymmetric dual-active sites for uncommon oxygen-evolving pathway.

Nature communications·2026
Same journal

Rapid GC content evolution in rice through GC-biased gene conversion and selection for translation efficiency.

Nature communications·2026
Same journal

Declines in organic matter persistence with increased soil carbon.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
11:34

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Published on: December 20, 2013

7.9K

Ultrastable cellulosome-adhesion complex tightens under load.

Constantin Schoeler1, Klara H Malinowska1, Rafael C Bernardi2

  • 1Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität, 80799 Munich, Germany.

Nature Communications
|December 9, 2014
PubMed
Summary
This summary is machine-generated.

Certain cellulosome interactions are exceptionally strong, resisting significant force. This discovery aids in developing robust biocatalysts for renewable biomass conversion.

More Related Videos

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

9.0K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

7.1K

Related Experiment Videos

Last Updated: Apr 19, 2026

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
11:34

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Published on: December 20, 2013

7.9K
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

9.0K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

7.1K

Area of Science:

  • Biochemistry
  • Biophysics
  • Microbiology

Background:

  • Cellulosomes are multi-component protein networks produced by specialized bacteria for lignocellulosic biomass digestion.
  • These networks rely on protein interactions for assembly and function.
  • Nature has evolved ultrastable protein complexes in response to challenging environments.

Purpose of the Study:

  • To characterize the ligand-receptor complex responsible for substrate anchoring in the Ruminococcus flavefaciens cellulosome.
  • To investigate the mechanical properties and force resistance of this specific cellulosomal interaction.
  • To understand the role of mechanical force in cellulosome complex stability and function.

Main Methods:

  • Single-molecule force spectroscopy was employed to measure the force resistance of the interaction.
  • Steered molecular dynamics simulations were used to analyze the complex's behavior under force.
  • Biochemical and biophysical techniques were utilized to characterize the ligand-receptor complex.

Main Results:

  • The characterized ligand-receptor complex exhibits extreme resistance to applied force, withstanding 600-750 pN.
  • This interaction strength is among the strongest reported bimolecular interactions, approaching the mechanical strength of a covalent bond.
  • Evidence of force activation and inter-domain stabilization of the complex was observed.

Conclusions:

  • Certain cellulosomal ligand-receptor interactions possess remarkable mechanical stability.
  • These strong interactions are crucial for maintaining the integrity of the cellulosome network.
  • Understanding these mechanical properties can inform the design of advanced biocatalysts for biomass conversion.