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Updated: Apr 19, 2026
Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Saturation mutagenesis in selected amino acids to shift Pseudomonas sp. acidic lipase Lip I.3 substrate specificity
Paola Panizza1, Silvia Cesarini, Pilar Diaz
1Bioscience Department, Facultad de Química, Universidad de la República (UdelaR), Montevideo, Uruguay. ppanizza@fq.edu.uy.
Abstract:
Several Pseudomonas sp. CR611 Lip I.3 mutants with overall increased activity and a shift towards longer chain substrates were constructed. Substitution of residues Y29 and W310 by smaller amino acids provided increased activity on C18-substrates. Residues G152 and S154, modified to study their influence on interfacial activation, displayed a five and eleven fold increased activity.
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