Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation.

Molecular therapy. Oncology·2026

Same author

Development-related gene expression disruption in the human midcingulate cortex in Huntington's disease.

Journal of Huntington's disease·2026

Same author

Evaluating the Effectiveness of the Motivational Interviewing-Based Wellness Coaching for Kids and Families (WC4K) Program in Pediatric Obesity Care: Protocol for a Cluster Randomized Pragmatic Trial.

JMIR research protocols·2026

Same author

Multiparametric Single-Vesicle Flow Cytometry Resolves Extracellular Vesicle Heterogeneity and Reveals Selective Regulation of Biogenesis and Cargo Distribution.

ACS nano·2024

Same author

Playgrounds Location and Patterns of Use.

Research square·2023

Same author

Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs.

Nano today·2023

Same journal

Analytical performance of poly(L-cysteine) modified pencil graphite electrode for dapsone detection.

Journal of pharmacological and toxicological methods·2026

Same journal

Corrigendum to "Nonclinical cardiovascular safety assessment of thioridazine: Impact of heart rate, body temperature, and choice of species" [Journal of Pharmacological and Toxicological Methods, Volume 115 (2022), 107167].

Journal of pharmacological and toxicological methods·2026

Same journal

Rapid and sensitive quantification of pemetrexed in human plasma by LC-MS/MS using a stable isotope-Labeled internal standard for therapeutic drug monitoring.

Journal of pharmacological and toxicological methods·2026

Same journal

The INSPIRE doctoral network in safety pharmacology - Looking back and ahead.

Journal of pharmacological and toxicological methods·2026

Same journal

NADES-assisted sustainable extraction of total protein content from Achyranthes bidentata seeds and their biochemical characterization.

Journal of pharmacological and toxicological methods·2026

Same journal

Non-invasive, home cage digital monitoring for improved safety pharmacology assessments in drug development: ICH S7A update considerations.

Journal of pharmacological and toxicological methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

A novel bicistronic sensor vector for detecting caspase-3 activation.

Tatyana Vagner¹, Alexandre Mouravlev², Deborah Young²

¹Centre for Brain Research, University of Auckland, 85 Park Rd, Grafton, Auckland 1142, New Zealand; Dept. of Molecular Medicine & Pathology, University of Auckland, 85 Park Rd, Grafton, Auckland 1142, New Zealand.

Journal of Pharmacological and Toxicological Methods

|December 9, 2014

Summary

This summary is machine-generated.

Researchers developed a novel bicistronic caspase-3 sensor for early apoptosis detection in single cells. This sensitive tool aids in understanding disease progression and timing therapeutic interventions.

Keywords:

Caspase-3 activation Caspase-3 sensor Cell-based assay Single cell detection

More Related Videos

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

Related Experiment Videos

Last Updated: Apr 19, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Detecting Anastasis In Vivo by CaspaseTracker Biosensor

Published on: February 1, 2018

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Apoptosis, or programmed cell death, is implicated in numerous human diseases.
Caspase-3 activation is a key biochemical marker for apoptosis.
Early detection of caspase-3 activation is crucial for timely intervention to prevent cell damage.

Purpose of the Study:

To develop a novel bicistronic caspase-3 sensor vector for detecting caspase-3 activation at the single-cell level.
To enhance sensitivity compared to traditional biochemical assays for apoptosis detection.

Main Methods:

Utilized green fluorescent protein (GFP) as a reporter for caspase-3 activity.
Constructs were tested in Neuro2A and HEK293 cells, inducing apoptosis with okadaic acid (OA) or staurosporine (STS).
Assessed sensor functionality by co-transfection with active caspase-3 and quantified GFP fluorescence.

Main Results:

Observed increased GFP expression in cells transfected with the bicistronic sensor upon OA and STS treatment.
Demonstrated a significant rise in GFP fluorescence intensity when co-expressing the sensor with active caspase-3.

Conclusions:

Successfully generated a novel bicistronic caspase-3 sensor vector using a transcription factor/response element system.
The sensor offers high sensitivity for single-cell detection and enables automated quantification of caspase-3 activation.