Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...

Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Proximity Labeling Reveals RNA-Binding Proteins Associating with the Human Mitochondrial Import Receptor TOMM20.

Journal of proteome research·2025

Same author

Expression of transgenic biotin ligases in inducible neuronal murine cell lines by integration into the mHipp11 gene locus.

PloS one·2025

Same author

The high-density lipoprotein binding protein HDLBP is an unusual RNA-binding protein with multiple roles in cancer and disease.

RNA biology·2024

Same author

Proteomic Mapping by APEX2-Catalyzed Proximity Labeling in Saccharomyces cerevisiae Semipermeabilized Cells.

Methods in molecular biology (Clifton, N.J.)·2022

Same author

RNA Proximity Labeling: A New Detection Tool for RNA-Protein Interactions.

Molecules (Basel, Switzerland)·2021

Same author

The Motor Neuron-Like Cell Line NSC-34 and Its Parent Cell Line N18TG2 Have Glycogen that is Degraded Under Cellular Stress.

Neurochemical research·2021

Same journal

Illuminating ribosome biogenesis disorders through structural biology.

RNA biology·2026

Same journal

Alpha-like subunits of multisubunit RNA polymerases: insights into structure, function, and disease.

RNA biology·2026

Same journal

RNA polymerase II phosphorylation dynamics: from molecular mechanisms to human disease.

RNA biology·2026

Same journal

Impact of interspecies colostrum and milk replacement on circulating sncRNA dynamics of neonatal goat kids.

RNA biology·2026

Same journal

The role of RNA modifications in cancer translational control.

RNA biology·2026

Same journal

Discovery of a mutation-containing circRNA in polyglutamine disease through systematic analysis of RNAs with CAG repeats.

RNA biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

Here, there, everywhere. mRNA localization in budding yeast.

Birgit Singer-Krüger¹, Ralf-Peter Jansen

¹a Interfaculty Institute of Biochemistry ; University of Tübingen ; Tübingen , Germany.

|December 9, 2014

Summary

This summary is machine-generated.

Messenger RNA (mRNA) localization in yeast reveals diverse pathways for protein sorting and cell polarity. This study uses Saccharomyces cerevisiae to analyze mRNA transport mechanisms and their physiological roles.

Keywords:

ASH1 Myo4p Saccharomyces cerevisiae She2p endoplasmic reticulum mitochondria polarity

More Related Videos

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Published on: July 19, 2021

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

Related Experiment Videos

Last Updated: Apr 19, 2026

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Published on: July 19, 2021

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization smFISH Analysis in Budding Yeast Vegetative Growth and Meiosis

Published on: May 25, 2018

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

mRNA localization and localized translation are crucial for cell polarity and asymmetry in eukaryotes.
In metazoans, mRNA transport is vital for embryonic development and neuronal function.
The yeast Saccharomyces cerevisiae serves as a model organism due to its simpler mRNA localization machinery.

Purpose of the Study:

To investigate the diverse mechanisms of mRNA localization in Saccharomyces cerevisiae.
To understand the role of mRNA localization in protein sorting to organelles.
To analyze the contribution of mRNA localization to cellular physiology and polarity establishment.

Main Methods:

Studying motor protein-dependent trafficking of translationally silenced mRNAs.
Investigating co-translational targeting of mRNAs on ribosomes.
Utilizing Saccharomyces cerevisiae as a model system for systemic analysis.

Main Results:

Identified diverse pathways for mRNA targeting to specific cellular destinations.
Demonstrated mRNA localization's role in protein sorting and organelle function.
Confirmed mRNA localization's importance in establishing and maintaining cell polarity.

Conclusions:

mRNA localization is a fundamental process with diverse mechanisms in yeast.
Understanding these pathways in yeast provides insights into fundamental cellular processes.
mRNA localization significantly contributes to cellular physiology, including polarity and protein sorting.