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Isoenzymes profiles in slowly-growing and difficult-to-grow mycobacteria
M C Blom-Potar1, H L David, N Rastogi
1Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
Acta Leprologica
|January 1, 1989
Abstract:
The cell-free extracts of 13 slowly-growing mycobacteria were run on polyacrilamide gels (Page) and the various protein bands obtained were tested for peroxidase and catalase enzyme activities. The results obtained were compared to those obtained with M. fortuitum and M. chelonae. Based only on a limited numbers of strains employed, it is suggested that these isoenzyme patterns may permit a better separation of "Wood-pigeon" mycobacteria from both M. avium and M. paratuberculosis and also give distinct profiles for other species used. These results further suggest the potential of isoenzymes as taxonomic markers.