Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying Structure-Property Relationships in Ferroelectric Polymers Toward High-Performance Soft Robots.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Dissecting the small RNA code of inflammatory bowel disease.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Paternal Microplastic Exposure Alters Sperm Small Noncoding RNAs and Affects Offspring Metabolic Health in Mice.

Journal of the Endocrine Society·2026
Same author

Conserved shifts in sperm small non-coding RNA profiles during mouse and human aging.

The EMBO journal·2026
Same author

Stage-Specific Drivers of Carbon-Sequestration Dynamics in <i>Porphyra</i> Mariculture and Responses to Global Warming.

Environmental science & technology·2025
Same author

A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis development.

Nature communications·2025

Related Experiment Video

Updated: Apr 19, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

3.5K

Cell electroporation with a three-dimensional microelectrode array on a printed circuit board.

Youchun Xu1, Shisheng Su1, Changcheng Zhou2

  • 1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.

Bioelectrochemistry (Amsterdam, Netherlands)
|December 9, 2014
PubMed
Summary

This study introduces a low-cost printed circuit board (PCB) chip for efficient cell electroporation. This novel method offers high transfection efficiency and reduced costs for biomedical research applications.

Keywords:
ElectroporationPCBThree-dimensionalTransfection

More Related Videos

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.2K
High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

707

Related Experiment Videos

Last Updated: Apr 19, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

3.5K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.2K
High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

707

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Electroporation facilitates molecule delivery into cells.
  • Microchip electroporation offers efficiency but requires complex fabrication.
  • A need exists for affordable and effective cell electroporation methods.

Purpose of the Study:

  • To develop a novel, cost-effective cell electroporation chip using standard printed circuit board (PCB) technology.
  • To evaluate the performance of PCB-fabricated electrodes for cell transfection.

Main Methods:

  • Fabricated an interdigitated electrode chip using standard PCB technology.
  • Conducted numerical simulations to compare 3D PCB electrodes with 2D planar electrodes.
  • Performed high-throughput electroporation experiments on multiple cell lines (HeLa, MCF7, COS7, Jurkat, 3T3-L1) with pEGFP-N1 plasmid.

Main Results:

  • PCB technology yielded thicker (35 μm) 3D electrodes with smooth edges.
  • Simulations indicated more uniform electric fields and reduced electrolysis with 3D electrodes.
  • Achieved efficient transfection across diverse cell lines using optimized parameters on the 18-well chip.

Conclusions:

  • Standard PCB technology provides a viable, low-cost alternative for fabricating effective electroporation chips.
  • The developed chip enables convenient, rapid, and high-throughput cell transfection.
  • This approach holds promise for affordable, disposable devices in biomedical research.