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Updated: Apr 19, 2026
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
Published on: April 24, 2018
In situ serial Laue diffraction on a microfluidic crystallization device
Sarah L Perry1, Sudipto Guha2, Ashtamurthy S Pawate2
1Department of Chemical Engineering, The University of Massachusetts Amherst , Amherst, MA, USA ; Institute for Molecular Engineering, The University of Chicago , Chicago, IL, USA ; Department of Chemical and Biomolecular Engineering, The University of Illinois at Urbana-Champaign , Urbana, IL, USA.
Abstract:
Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform for serial Laue diffraction from macroscopic crystals and proposes that a collection of small slices of Laue data from many individual crystals is a realistic solution to the difficulties in dynamic studies of irreversible biochemical reactions.
