Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.4K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.4K
Centrifugation01:05

Centrifugation

9.2K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
9.2K
Subcellular Fractionation01:32

Subcellular Fractionation

9.8K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infection following foot and ankle surgery : a subanalysis of data captured from the UK Foot and Ankle Thromboembolism (FATE) audit.

The bone & joint journal·2026
Same author

Postoperative pain after dental procedures: The National Dental Practice-Based Research Network observational study using an mobile health platform.

Journal of the American Dental Association (1939)·2026
Same author

Performance and Wellbeing Research Priorities in Premiership Women's Rugby: A Delphi Study Including Players and Staff.

European journal of sport science·2025
Same author

Isoform-specific vs. pan-histone deacetylase inhibition as approaches for countering glioblastoma: an <i>in vitro</i> study.

Frontiers in oncology·2025
Same author

Design, manufacture and evaluation of an offloading 3D printed wheelchair cushion.

Disability and rehabilitation. Assistive technology·2025
Same author

Clinical and cost-effectiveness of flexor digitorum profundus (FDP) versus FDP and flexor digitorum superficialis (FDS) repair for complete zone 2 flexor tendon injuries (FLARE): protocol for a randomised controlled trial.

Trials·2025

Related Experiment Video

Updated: Apr 19, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.2K

Separation of blood cells with differing deformability using deterministic lateral displacement(†).

David Holmes1, Graeme Whyte2, Joe Bailey3

  • 1London Centre for Nanotechnology , University College London , 17-19 Gordon Street, London WC1H 0AH , UK.

Interface Focus
|December 9, 2014
PubMed
Summary

Deterministic lateral displacement (DLD) offers a high-throughput, marker-free method to separate blood cells by mechanical properties. This technique successfully sorts cells based on stiffness and deformability, showing potential for leukocyte sub-population isolation.

Keywords:
blood separationcell deformabilitydeterministic lateral displacementdigital holographymicrofluidicsoptical stretching

More Related Videos

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.9K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.7K

Related Experiment Videos

Last Updated: Apr 19, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.2K
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.9K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.7K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Cell mechanical properties are crucial for characterization and separation.
  • Emerging techniques aim for marker-free cell analysis.
  • Deterministic Lateral Displacement (DLD) is a novel microfluidic approach.

Purpose of the Study:

  • To investigate the use of DLD for high-throughput blood cell separation based on mechanical phenotype.
  • To analyze the effect of altered membrane deformability on cell behavior in DLD devices.
  • To evaluate DLD's capability in isolating leukocyte sub-populations.

Main Methods:

  • Deterministic Lateral Displacement (DLD) microfluidic device.
  • Chemical modification of red blood cells to alter membrane stiffness.
  • Optical stretching for independent stiffness quantification.
  • Analysis of cell lateral displacement and exit positions within the DLD device.

Main Results:

  • Cell lateral displacement in DLD correlates with cell stiffness.
  • DLD device performance was validated using glutaraldehyde-cross-linked erythrocytes.
  • Successful isolation of leukocyte sub-populations (T-lymphocytes, neutrophils) based on size and deformability was demonstrated.
  • Leukocyte isolation efficiency varied with applied shear rate.

Conclusions:

  • DLD is an effective method for separating blood cells based on mechanical properties.
  • The technique shows promise for continuous fractionation and enrichment of specific leukocyte sub-populations from whole blood.
  • DLD offers a high-throughput, marker-free platform for cell analysis and sorting.