Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.2K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.2K
Determination of Crystal Structures01:29

Determination of Crystal Structures

126
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
126
X-ray Crystallography02:18

X-ray Crystallography

27.2K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
27.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insights into the solution structure of the actin-binding tail domain of metavinculin by small angle X-ray scattering and molecular dynamics simulations.

International journal of biological macromolecules·2025
Same author

Small angle X-ray scattering analysis of thermophilic cytochrome P450 CYP119 and the effects of the N-terminal histidine tag.

International journal of biological macromolecules·2024
Same author

Conformational multiplicity of bacterial ferric binding protein revealed by small angle x-ray scattering and molecular dynamics calculations.

The Journal of chemical physics·2023
Same author

Protein fibrillation from another small angle-SAXS data analysis of developing systems.

Methods in enzymology·2023
Same author

Protein fibrillation from another small angle: Sample preparation and SAXS data collection.

Methods in enzymology·2022
Same author

Identifying Biological and Biophysical Features of Different Maturation States of α-Synuclein Amyloid Fibrils.

Methods in molecular biology (Clifton, N.J.)·2022

Related Experiment Video

Updated: Apr 19, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.6K

Investigating increasingly complex macromolecular systems with small-angle X-ray scattering.

Bente Vestergaard1, Zehra Sayers2

  • 1Department of Drug Design and Pharmacology, University of Copenhagen , Universitetsparken 2, Copenhagen, DK-2100, Denmark.

Iucrj
|December 9, 2014
PubMed
Summary

Biological small-angle X-ray scattering (BioSAXS) has advanced significantly, enabling complex biological studies. This review highlights novel methods and recent results, showcasing the field's rapid growth and expanding capabilities.

Keywords:
beamlinesbiological solution small-angle X-ray scattering (BioSAXS)biostructural researchstructural complexitysynchrotron radiation

More Related Videos

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
12:53

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

18.9K
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

10.5K

Related Experiment Videos

Last Updated: Apr 19, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.6K
Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
12:53

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

18.9K
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

10.5K

Area of Science:

  • Structural biology
  • Biophysics
  • Biochemistry

Background:

  • Biological small-angle X-ray scattering (BioSAXS) has seen substantial advancements.
  • The method now addresses increasingly complex biological questions.
  • A growing user community drives further innovation.

Purpose of the Study:

  • To review recent developments in BioSAXS.
  • To highlight novel BioSAXS methodologies.
  • To present recent results from BioSAXS studies.

Main Methods:

  • Review of hardware and software advancements.
  • Analysis of data collection and evaluation strategies.
  • Integration with complementary techniques.

Main Results:

  • Significant results achieved through synergistic advances.
  • Rapid growth in the BioSAXS user community.
  • Increasingly ambitious research questions being addressed.

Conclusions:

  • BioSAXS is a powerful and evolving technique.
  • Synergies between different advancements drive progress.
  • The field is poised for continued expansion and impact.