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Related Experiment Video

Updated: Apr 19, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
15:32

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

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CD71(high) population represents primitive erythroblasts derived from mouse embryonic stem cells.

Ruihua Chao1, Xueping Gong1, Libo Wang1

  • 1Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China.

Stem Cell Research
|December 9, 2014
PubMed
Summary

High CD71 expression on differentiating embryonic stem cells (ESCs) identifies primitive erythroblasts (EryPs). This finding advances understanding of early red blood cell development and provides a method for isolating EryPs in vitro.

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Area of Science:

  • Hematology
  • Developmental Biology
  • Stem Cell Research

Background:

  • The CD71/Ter119 marker combination is standard for tracking red blood cell maturation in vivo.
  • CD71's expression on proliferating cells raises questions about its specificity during embryonic stem cell (ESC) differentiation.

Purpose of the Study:

  • To determine if CD71 can serve as an erythrocyte-specific marker during ESC differentiation.
  • To characterize the CD71(high) population during in vitro ESC differentiation.
  • To investigate the molecular regulation of primitive erythropoiesis in vitro.

Main Methods:

  • Mouse embryonic stem cells (ESCs) were differentiated in vitro.
  • Flow cytometry was used to identify and sort CD71(high) cells.
  • Analysis of globin switching and enucleation was performed.
  • Gene expression analysis of key regulatory factors (Scl, HoxB4, Eaf1, Klf1) was conducted.

Main Results:

  • A CD71(high) population during ESC differentiation mirrors in vivo yolk sac-derived primitive erythroblasts (EryPs).
  • These CD71(high) cells undergo maturational globin switching and enucleation, similar to EryPs.
  • The formation of the CD71(high) population is differentially regulated by Scl, HoxB4, Eaf1, and Klf1.

Conclusions:

  • CD71(high) expression is a reliable marker for identifying primitive erythroblasts during in vitro ESC differentiation.
  • This study establishes a method for efficient isolation of EryPs from differentiated ESCs.
  • The findings provide insights into the molecular mechanisms governing primitive erythropoiesis.