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Updated: Apr 19, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
Published on: February 17, 2017
Comparison of multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles
Minfeng Chen1, Pengfei Song2, Dan Zou3
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; BGI-Shenzhen, Shenzhen, 518083, China.
Abstract:
Single-cell sequencing promotes our understanding of the heterogeneity of cellular populations, including the haplotypes and genomic variability among different generation of cells. Whole-genome amplification is crucial to generate sufficient DNA fragments for single-cell sequencing projects. Using sequencing data from single sperms, we quantitatively compare two prevailing amplification methods that extensively applied in single-cell sequencing, multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC). Our results show that MALBAC, as a combination of modified MDA and tweaked PCR, has a higher level of uniformity, specificity and reproducibility.
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