Related Experiment Video
Updated: Apr 19, 2026
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Published on: December 3, 2011
Molecular detection of human adenovirus in sediment using a direct detection method compared to the classical
Rodrigo Staggemeier1, Marina Bortoluzzi1, Tatiana Moraes da Silva Heck1
1Laboratory of Molecular Microbiology, Universidade Feevale, ERS 239, No. 2755, Novo Hamburgo, Rio Grande do Sul 93352-000, Brazil.
Abstract:
Various effective methods have been developed to measure the concentration of viruses in sediment samples. However, there is need to standardize less laborious and simpler techniques. The objective of the present study was to compare two different methods to measure the concentration of viruses in soil samples. The use of polyethylene glycol (PEG) was compared with a direct extraction of viral nucleic acids from the samples diluted in modified Eagle's minimal essential medium (E-MEM). The presence of adenovirus in the samples was detected by real-time quantitative polymerase chain reaction (qPCR). Only six samples (30%) were positive for adenovirus when PEG technique was used. The direct method showed 16 (80%) samples positive for adenovirus. Therefore, direct detection (i.e. without previous concentration) demonstrated a higher rate of detection, better effectiveness, and shorter execution time. Furthermore, direct detection uses reagents that are often readily available in virology laboratories. Thus, it is an attractive alternative to other methods of detection of virus particles in sediments.
