Related Experiment Video
Updated: Apr 19, 2026

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
Published on: October 7, 2016
(1)H NMR z-spectra of acetate methyl in stretched hydrogels: quantum-mechanical description and Markov chain Monte
Dmitry Shishmarev1, Bogdan E Chapman1, Christoph Naumann1
1School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
Abstract:
The (1)H NMR signal of the methyl group of sodium acetate is shown to be a triplet in the anisotropic environment of stretched gelatin gel. The multiplet structure of the signal is due to the intra-methyl residual dipolar couplings. The relaxation properties of the spin system were probed by recording steady-state irradiation envelopes ('z-spectra'). A quantum-mechanical model based on irreducible spherical tensors formed by the three magnetically equivalent spins of the methyl group was used to simulate and fit experimental z-spectra. The multiple parameter values of the relaxation model were estimated by using a Bayesian-based Markov chain Monte Carlo algorithm.
Related Concept Videos
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei...
IR Frequency Region: X–H Stretching
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
¹H NMR of Labile Protons: Temporal Resolution
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the...
Spectroscopy of Carboxylic Acid Derivatives

