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Updated: Apr 19, 2026
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Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
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Screening ancient tuberculosis with qPCR: challenges and opportunities
Kelly M Harkins1, Jane E Buikstra1, Tessa Campbell2
1School of Human Evolution and Social Change, Arizona State University, Tempe, AZ, USA.
Summary
Quantitative PCR (qPCR) effectively screens ancient DNA (aDNA) samples for Mycobacterium tuberculosis complex (MTBC). This method identifies promising samples for further analysis, improving efficiency and reducing costs in ancient pathogen research.
Area of Science:
- Paleogenomics
- Molecular Biology
- Infectious Disease Epidemiology
Background:
- Ancient DNA (aDNA) research is advancing due to new sequencing and enrichment techniques.
- DNA preservation in ancient tissues, particularly for pathogens, presents significant challenges.
- Screening methods are crucial for cost-effective and efficient downstream analyses.
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