Related Experiment Videos
A rapid procedure to identify newborn transgenic mice
C S Lin1, T Magnuson, D Samols
1Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.
DNA (Mary Ann Liebert, Inc.)
|May 1, 1989
Summary
We developed a rapid, 1-day protocol to identify newborn transgenic mice using tail tissue. This method detects foreign genetic material efficiently for early-stage research applications.
Area of Science:
- Molecular Biology
- Genetics
- Animal Models
Background:
- Transgenic mouse models are crucial for genetic research.
- Early identification of transgenic animals is essential for efficient breeding and experimental design.
Purpose of the Study:
- To establish a rapid, single-day protocol for identifying newborn transgenic mice.
- To enable early detection of foreign genetic material in mouse genomes.
Main Methods:
- Utilizing collagenase digestion of small tail biopsies from newborn mice.
- Employing phenol-chloroform extraction for DNA isolation.
- Applying polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) for genetic analysis.
Main Results:
- Successful identification of transgenic mice within 1 day of tissue biopsy.
- The protocol is effective for detecting foreign genetic material in the mouse genome.
- The method allows for very early tissue sampling shortly after birth.
Conclusions:
- The developed protocol provides a fast and reliable method for early identification of transgenic mice.
- This 1-day procedure streamlines the process of generating and validating transgenic animal models.
- Efficient identification facilitates timely progression of genetic research and development.