Related Experiment Video
Updated: Apr 19, 2026

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
Published on: November 10, 2018
A novel posavirus-related single-stranded RNA virus from fish (Cyprinus carpio)
Gábor Reuter1, Péter Pankovics, Eric Delwart
1Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Szabadság út 7, 7623, Pécs, Hungary, reuter.gabor@ddr.antsz.hu.
Abstract:
The complete genome sequence of a novel +ssRNA virus, provisionally named fisavirus 1 (strain HAL1/fisa-ssRNAV, KM434233), identified from a freshwater carp (Cyprinus carpio), was determined using viral metagenomics and RT-PCR. The 8712-nt-long genome of HAL1/fisa-ssRNAV shares the same genome organization (i.e., a potential single ORF and N-terminal Hel-Pro-Pol replication domains followed by the structural proteins) and distant phylogenetic relationship to the currently unclassified posaviruses, which suggests that these viruses could belong to different genera in a novel family of the order Picornavirales.
Related Concept Videos
Rous Sarcoma Virus (RSV) and Cancer
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer
Viruses with RNA Genomes
Subviral Agents
Hepatitis
Viruses of Archaea

