Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

3.8K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
3.8K
Antibiotic Selection00:57

Antibiotic Selection

62.4K
Overview
62.4K
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

2.8K
Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
2.8K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.4K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyanobacterial flocculation as a defence against bacterial predation.

The ISME journal·2026
Same author

From pond to platform: how <i>Synechocystis</i> sp. PCC 6803 became the default model cyanobacterium.

Journal of bacteriology·2026
Same author

Non-linear frequency doubling up-conversion of geothermal radiation cannot provide sufficient light to support oxygenic photosynthesis at deep-sea hydrothermal vents.

National science review·2025
Same author

Beyond movement: the dynamic roles of Type IV pili in cyanobacterial life.

Journal of bacteriology·2025
Same author

The RRM domain-containing protein Rbp3 interacts with ribosomes and the 3' ends of mRNAs encoding photosynthesis proteins.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Does mRNA targeting explain gene retention in chloroplasts?

Trends in plant science·2024

Related Experiment Video

Updated: Apr 19, 2026

Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans
07:05

Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans

Published on: August 17, 2022

4.0K

Bacteria in solitary confinement.

Conrad W Mullineaux1

  • 1School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom; Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Freiburg, Germany c.mullineaux@qmul.ac.uk.

Journal of Bacteriology
|December 10, 2014
PubMed
Summary
This summary is machine-generated.

Individual bacteria exhibit significant stochastic variation, complicating data interpretation. A novel microfluidic device allows gentle isolation of single cells for studying environmental responses, like osmoadaptation in cyanobacteria.

More Related Videos

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

3.1K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.5K

Related Experiment Videos

Last Updated: Apr 19, 2026

Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans
07:05

Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans

Published on: August 17, 2022

4.0K
In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

3.1K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.5K

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Clonal bacterial cultures can display significant stochastic variation among individual cells.
  • This cellular heterogeneity poses challenges for interpreting bulk culture data.
  • Understanding single-cell responses is crucial for accurate biological insights.

Purpose of the Study:

  • To introduce and validate a novel microfluidic device for isolating individual bacterial cells.
  • To enable the study of osmoadaptation in a cyanobacterium at the single-cell level.
  • To provide a new tool for probing cellular responses to environmental stimuli.

Main Methods:

  • Development of an ingenious microfluidic device designed for gentle cell caging.
  • Application of the device to study osmoadaptation in a cyanobacterium.
  • Observation and analysis of individual cell behavior within the microfluidic environment.

Main Results:

  • The microfluidic device successfully isolates individual bacterial cells without causing harm.
  • The study provides insights into the osmoadaptation process of a cyanobacterium at the single-cell level.
  • The device demonstrates utility in observing stochastic variations in cellular responses.

Conclusions:

  • The developed microfluidic device is a valuable addition to the available tools for single-cell analysis.
  • This technology facilitates the investigation of cellular heterogeneity and responses to environmental cues.
  • The approach aids in overcoming interpretation pitfalls associated with bulk bacterial culture data.