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Related Concept Videos

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Related Experiment Video

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Quantitative polymerase chain reaction

James R C Miller1, Ralph Andre

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|December 10, 2014
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No abstract available in PubMed .

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