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Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

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Related Experiment Video

Updated: Apr 19, 2026

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Production methods for viral particles.

Kodai Machida¹, Hiroaki Imataka

¹Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Himeji, 671-2201, Japan, machida@eng.u-hyogo.ac.jp.

Biotechnology Letters

|December 10, 2014

Summary

This summary is machine-generated.

Viral particles and virus-like particles (VLPs) are versatile tools for bio-imaging and drug delivery. This review covers methods for producing and functionalizing these particles for scientific applications.

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Area of Science:

Biotechnology and Nanotechnology
Biomaterials Science

Background:

Viral particles and virus-like particles (VLPs) are increasingly utilized in bio-imaging, drug delivery, and materials science.
Their unique structure and versatility make them attractive for various advanced applications.

Purpose of the Study:

To review and compare different methods for producing viral particles and VLPs.
To discuss techniques for functionalizing these particles for specific scientific uses.
To evaluate the advantages and disadvantages of various production and functionalization systems.

Main Methods:

Production of viral particles via host organism infection.
Production of VLPs through expression of capsid proteins in cells.
Reassembly of disassembled VLPs to incorporate specific materials.
Cell-free systems for controlled viral particle production.
Genetic and chemical engineering for particle functionalization.

Main Results:

Various production systems exist, each with unique benefits and limitations.
Functionalization strategies are crucial for tailoring particles to specific applications.
Cell-free systems offer enhanced control over the assembly process.

Conclusions:

Viral particles and VLPs are adaptable platforms for diverse scientific fields.
Understanding production and functionalization methods is key to maximizing their potential.
Continued research in these areas will drive innovation in biotechnology and materials science.