Related Experiment Video

Updated: Apr 19, 2026

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
09:38

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Published on: August 11, 2017

9.3K

Relationship of thyroid transcription factor 1 to EGFR status in non-small-cell lung cancer

B S Sheffield1, I E Bosdet1, R H Ali2

  • 1Department of Pathology and Laboratory Medicine, BC Cancer Agency. Vancouver, BC.

Current Oncology (Toronto, Ont.)
|December 10, 2014
PubMed
Abstract

Insights

Thyroid transcription factor 1 (TTF1) immunohistochemistry can predict epidermal growth factor receptor (EGFR) status in non-small-cell lung cancer. TTF1 negativity by IHC is highly predictive of negative EGFR status, aiding treatment decisions.

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Cancer Biomarkers

Background:

  • Activating mutations in the epidermal growth factor receptor (EGFR) gene drive a subset of non-small-cell lung cancers (NSCLC).
  • Identifying EGFR mutations in NSCLC patients enables targeted therapy with EGFR-blocking agents.
  • Accurate EGFR mutation status is crucial for effective NSCLC treatment strategies.

Purpose of the Study:

  • To evaluate the utility of thyroid transcription factor 1 (TTF1) immunohistochemistry (IHC) as a surrogate marker for EGFR mutation status in NSCLC.
  • To determine if TTF1 IHC can aid in optimizing EGFR testing strategies, especially in cases with limited tissue.
  • To assess the correlation between TTF1 negativity and EGFR mutation status in a clinical setting.

Main Methods:

  • Prospective collection and cataloging of all specimens submitted for EGFR testing at BC Cancer Agency over 14 months.
  • Analysis of EGFR test results and associated clinical information.
  • Concurrent immunohistochemical (IHC) analysis for TTF1 on available specimens.

Main Results:

  • EGFR status was reported for 509 out of 586 patient specimens received for testing.
  • No significant relationship was found between specimen type/origin and EGFR test failure rates.
  • TTF1-negative status by IHC demonstrated a high negative predictive value (94.2%) for predicting negative EGFR status.

Conclusions:

  • Immunohistochemistry for TTF1 is a readily available and reliable surrogate marker for EGFR status in NSCLC.
  • TTF1 IHC can help optimize EGFR testing strategies, particularly when tissue is limited, facilitating timely patient management.
  • Combining TTF1 IHC with genetic assays can enhance the efficiency of EGFR testing in NSCLC.
Keywords:
EGFRadenocarcinomabiomarker testinglung cancernon-small-cell lung cancernsclcthyroid transcription factor 1ttf1

More Related Videos

Induction and Analysis of Epithelial to Mesenchymal Transition10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

36.8K
Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

890

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.5K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.1K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
21.3K
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
74
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
11.1K