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Methods to Assess Microbial Communities

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Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...

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Technique for Studying Arthropod and Microbial Communities within Tree Tissues

Technique for Studying Arthropod and Microbial Communities within Tree Tissues

Published on: November 16, 2014

Technique for studying arthropod and microbial communities within tree tissues.

Nicholas C Aflitto¹, Richard W Hofstetter², Reagan McGuire¹

¹School of Forestry, Northern Arizona University.

Journal of Visualized Experiments : Jove

|December 10, 2014

Summary

Researchers developed a

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A Method for Quantifying Foliage-Dwelling Arthropods

A Method for Quantifying Foliage-Dwelling Arthropods

Published on: October 20, 2019

Area of Science:

Entomology
Microbiology
Botany

Background:

Pine phloem and cambium tissues serve as crucial microhabitats for diverse arthropod and microbial communities.
Observing these organisms within their natural phloem habitat is challenging due to their sub-bark location.

Purpose of the Study:

To present an innovative technique for preserving intact phloem tissue.
To facilitate the study of invertebrates and microorganisms within the phloem environment.

Main Methods:

Introduction of the 'phloem sandwich' apparatus, designed for the introduction and observation of organisms.
The technique allows for the preservation of phloem integrity and flexibility in organism introduction.

Main Results:

The phloem sandwich method enables detailed observation of feeding behaviors, life-history traits, reproduction, development, and interactions of phloem-dwelling organisms.
This technique enhances the understanding of ecological dynamics within tree phloem.

Conclusions:

The phloem sandwich is a cost-effective, versatile, and valuable tool for scientific research and education.
It supports the study of forest ecosystems and organismal interactions in a unique habitat.