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Β-catenin is involved in Bex2 down-regulation induced glioma cell invasion/migration inhibition

Er Nie1, Xu Zhang2, Shao Xie2

  • 1The Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu, China.

Biochemical and Biophysical Research Communications
|December 10, 2014
PubMed

Insights

Brain expressed X-linked gene 2 (Bex2) regulates glioma cell invasion and migration by controlling β-catenin protein levels. Down-regulating Bex2 reduces glioma cell invasion and migration via decreased β-catenin.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Brain expressed X-linked gene 2 (Bex2) has been previously identified as a regulator of glioma cell invasion and migration.
  • The precise molecular mechanisms underlying Bex2's role in glioma progression remain largely unelucidated.

Purpose of the Study:

  • To investigate the mechanism by which Bex2 influences glioma cell invasion and migration.
  • To determine the relationship between Bex2 and β-catenin in glioma tissues and cells.

Main Methods:

  • Analysis of Bex2 and β-catenin protein and mRNA levels in glioma tissues and cells.
  • Investigating the subcellular localization of β-catenin following Bex2 down-regulation.
  • Functional rescue experiments involving β-catenin over-expression.

Main Results:

  • Bex2 down-regulation significantly inhibited glioma cell migration and invasion.
  • Bex2 down-regulation led to decreased protein levels of β-catenin in the nucleus and cytoplasm, but not mRNA levels.
  • Over-expression of β-catenin reversed the inhibitory effects of Bex2 down-regulation on glioma cell invasion and migration.

Conclusions:

  • Bex2 regulates glioma cell invasion and migration by modulating β-catenin protein levels.
  • The findings highlight a novel regulatory pathway involving Bex2 and β-catenin in glioma progression.
Keywords:
Bex2GlioblastomaInvasionMigrationβ-Catenin

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